• 10.1021/ac100101d
  • Analytical Chemistry
  • Volume 82
  • June 2010
  • pp 4403-4412

LC-MS method for core metabolites; readily implemented.


Topic Area
Biology / biological chemistry
Article Type
Primary Research

Logic / Design


Point of Paper
Very clear
Experimental Design
Excellent
Main Conclusions
Supported by evidence presented

Reproducibility


Key Procedures
Able to reproduce all
# Procedures Attempted
1 to 3
Of Attempted, # Reproduced
All

Improvements


Replicated Methods Exactly:
Exactly
Reviewer Improvements
No improvements made

Versatility


Applicability to Other Fields
Definitely
Use in Own Work
Yes, and it was useful

Clarity


Overall Clarity
Exceptionally clear & well-written

Impact


Impact
Signifcantly advances the field

Transparency


Funding Sources
Clearly disclosed
Conflicts of Interest
Not aware of any

Other


Safety Concerns
None or clearly mentioned

Details


A method for LCMS analysis and quantification of core metabolites (e.g. NADH/NAD, glycolysis, citrate cycle, etc.) was presented by the authors. I established their method on our own device, which worked well and only required the device-specific typical optimisation (here, mass spectrometry experimental parameters, the liquid chromatography part could directly be implemented). The method worked straight away and was robust for analysis.



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