Preparation and characterisation of fluorescent chitosans using 9-anthraldehyde as fluorophore

Quality paper, good reproducibility


The procedure was adapted for the covalent attachment of an organometallic complex to chitosan backbone for use in a glucose oxidase bioanode. The procedure was done in both acidic (as in paper) and basic conditions (for comparison) for the following reasons: acidic media provided solubility for the chitosan, but basic media offered the advantages of enhancing the nucleophilicity of the amine residues within the chitosan. In both cases, the 50% methanol allowed for the dissolution of the metal complex. Both experiments yielded successful reductive amination in redox-active polymer. Yields were higher in basic media; while the chitosan is unable to dissolve in basic solutions, it can swell to create a porous matrix, allowing for more sites on the polymer to be accessed. Yields were high as reported (about 80% and 90% for acidic and basic media, respectively). For application purposes, lower quantities of organometallic species were used to keep the redox polymer soluble.

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