Papers

With Tag: Biochemical Methods

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Bioprobes Based on AIE Fluorogens

Kai Li, Dan Ding, Ben Zhong Tang, Bin Liu
Acc. Chem. Res. | 2013 | 10.1021/ar3003464

Fluorescent bioprobes are powerful tools for analytical sensing and optical imaging, which allow direct visualization of biological analytes at the molecular level and offer useful insights into complex biological structures and processes. The sensing and...

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Measuring Uptake Dynamics of Multiple Identifiable Carbon Nanotube Species via High-Speed Confocal Raman Imaging of Live Cells

Daniel A. Heller, Ramachandra R. Dasari, Freddy T. Nguyen, Jeon Woong Kang, Niyom Lue
Nano Lett. | 2012 | 10.1021/nl302991y

Carbon nanotube uptake was measured via high-speed confocal Raman imaging in live cells. Spatial and temporal tracking of two cell-intrinsic and nine nanotube-derived Raman bands was conducted simultaneously in RAW 264.7 macrophages. Movies resolved single...

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CdSe−ZnS Quantum Dots as Resonance Energy Transfer Donors in a Model Protein−Protein Binding Assay

Dale M. Willard, Jaemyeong Jung, Alan Van Orden, Lori L. Carillo
Nano Letters | 2001 | 10.1021/nl010068s

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Patterned DNA Metallization by Sequence-Specific Localization of a Reducing Agent

Rotem S. Berman, Kinneret Keren, Erez Braun
Nano Letters | 2004 | 10.1021/nl035124z

Localization of a reducing agent, glutaraldehyde, on DNA molecules directs their metallization into highly conductive wires. DNA can be marked for metallization by aldehyde derivatization while retaining its biological functionality. Patterning the aldehyde...

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Detection and Size Analysis of Proteins with Switchable DNA Layers

Erika Pringsheim, Naoki Yokoyama, Ulrich Rant, Jelena Knezevic, Gerhard Abstreiter, Shozo Fujita, Wolfgang Kaiser, Marc Tornow, Kenji Arinaga
Nano Lett. | 2009 | 10.1021/nl8026789

We introduce a chip-compatible scheme for the label-free detection of proteins in real-time that is based on the electrically driven conformation switching of DNA oligonucleotides on metal surfaces. The switching behavior is a sensitive indicator for the...

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Dynamically Configurable Biomolecular Nanoarrays

Yong Chen, Suxian Huang, Eric Schopf
Nano Lett. | 2007 | 10.1021/nl071643g

Nanoarrays of distinct DNA and protein biomolecules were fabricated by electrochemically controlling their assembly/release from Au nanoelectrodes on a chip. The surface density, ratio, and activity of the biomolecules assembled on each nanoelectrode in the...

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Synthesis and Thermally Reversible Assembly of DNA−Gold Nanoparticle Cluster Conjugates

Jae-Seung Lee, Ji-Young Kim
Nano Lett. | 2009 | 10.1021/nl9030709

We describe the facile synthesis of stable gold nanoparticle clusters densely functionalized with DNA (DNA−AuNP clusters) using dithiothreitol and monothiol DNA and their thermally reversible assembly properties. The size of the clusters exhibits a very...

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Dependence of Fluorescence Intensity on the Spectral Overlap between Fluorophores and Plasmon Resonant Single Silver Nanoparticles

David S. Ginger, Keiko Munechika, Yeechi Chen
Nano Lett. | 2007 | 10.1021/nl062795z

We investigate the fluorescence from dyes coupled to individual DNA-functionalized metal nanoparticles. We use single-particle darkfield scattering and fluorescence microscopy to correlate the fluorescence intensity of the dyes with the localized surface...

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Development and Evaluation of an Online CO2 Evolution Test and a Multicomponent Biodegradation Test System

Applied and Environmental Microbiology | 2004 | 10.1128/AEM.70.8.4621-4628.2004

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A Suite of 2H NMR Spin Relaxation Experiments for the Measurement of RNA Dynamics

Pramodh Vallurupalli, Lewis E. Kay
Journal of American Chemical Society | 2005 | 10.1021/ja0427799

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Relaxation Artifacts and Their Suppression in Multidimensional E.COSY-type NMR Experiments for Measurement of J Coupling Constants in 13C- or 15N-Labeled Proteins

Axel Meissner, Thomas Schulte-Herbrüggen, Ole Winneche Sørensen
Journal of American Chemical Society | 1998 | 10.1021/ja9730371

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Genetic Engineering Combined with Deep UV Resonance Raman Spectroscopy for Structural Characterization of Amyloid-like Fibrils

Vitali Sikirzhytski, Natalya I. Topilina, Seiichiro Higashiya, John T. Welch, Igor K. Lednev
Journal of American Chemical Society | 2008 | 10.1021/ja8006275

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An Efficient Computational Method for Predicting Rotational Diffusion Tensors of Globular Proteins Using an Ellipsoid Representation

Yaroslav E. Ryabov, Charles Geraghty, Amitabh Varshney, David Fushman
Journal of American Chemical Society | 2006 | 10.1021/ja062715t

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Mask-Directed Multiphoton Lithography

Bryan Kaehr, Jason B. Shear
Journal of American Chemical Society | 2007 | 10.1021/ja068390y

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Identification of Bacteria by Conjugated Oligoelectrolyte/Single-Stranded DNA Electrostatic Complexes

Aidee Duarte, Arkadiusz Chworos, Suvi F. Flagan, Grady Hanrahan, Guillermo C. Bazan
Journal of American Chemical Society | 2010 | 10.1021/ja105747b

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Quantitating Cell–Cell Interaction Functions with Applications to Glioblastoma Multiforme Cancer Cells

Qiongyu Shi, Jiashu Yu, Chentai Ma, Habib Ahmad, W Wei, Junfeng Wang, Young Shik Shin, Heath, R D Levine, D Tham
Nano Letters | 2012 | 10.1021/nl302748q

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Web-based resources for mass-spectrometry-based metabolomics: a user's guide.

Alisdair R Fernie, Takayuki Tohge
Phytochemistry | 2009 | 10.1016/j.phytochem.2009.02.004

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