Papers

With Tag: Biochemical Methods

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Reversible Electrochemical Detection of Nonelectroactive Polyions

Eric Bakker, Alexey Shvarev
J. Am. Chem. Soc. | 2003 | 10.1021/ja037167n

Selective extraction principles for the recognition of nonelectroactive polyions such as heparin and protamine exist, but the high ionic valency renders the extraction process irreversible. A response principle for the reversible detection of such polyions is...

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Site-Specific Protein Immobilization by Staudinger Ligation

Kimberly A. Dickson, Ronald T. Raines, Bradley L. Nilsson, Matthew B. Soellner
J. Am. Chem. Soc. | 2003 | 10.1021/ja036712h

The Staudinger ligation between an azido-protein and a phosphinothioester-derivatized surface is demonstrated to be an effective means for the site-specific, covalent immobilization of a protein. Immobilization yields of >50% are obtained in <1 min, and...

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Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates

Pablo D. Jadzinsky, Christopher J. Ackerson, Grant J. Jensen, Roger D. Kornberg
J. Am. Chem. Soc. | 2006 | 10.1021/ja0555668

A general method of rigid, specific labeling of proteins with gold clusters has been devised. The method relies on the conjugation of a glutathione monolayer-protected gold cluster (MPC) with a single chain Fv antibody fragment (scFv), mutated to present an...

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Tuning the Orientation of an Antigen by Adsorption onto Nanostriped Templates

Françoise Blondeau, Benoit De Meersman, Bernard Nysten, Alain M. Jonas, Antoine Pallandre
J. Am. Chem. Soc. | 2005 | 10.1021/ja043656r

We investigate the adsorption of a globular protein (P.69 pertactin, also known as antigen 69k) on protein-repellent hydrophilic substrates bearing regularly spaced hydrophobic nanostripes, for stripe widths comprised between 20 and 160 nm. Protein adsorption...

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Merocyanine Dyes with Improved Photostability

Dan-Vinh Nguyen, Alexei Toutchkine, Klaus M. Hahn
Org. Lett. | 2007 | 10.1021/ol070780h

Merocyanine dyes have proven valuable for live cell fluorescence imaging applications, but many structures have been limited by rapid photobleaching. We show that photostability is substantially enhanced for merocyanines having a cyano group at a specific...

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Biochemical transformation of coals

1999

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Spin-State-Selective Polarization or Excitation for Simultaneous E.COSY-Type Measurement of 3J(C‘,Hα) and 3J(HN,Hα) Coupling Constants with Enhanced Sensitivity and Resolution in Mul

Axel Meissner, Thomas Schulte-Herbrüggen, Ole Winneche Sørensen
Journal of American Chemical Society | 1998 | 10.1021/ja973358c

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NMR Characterization of Membrane Protein−Detergent Micelle Solutions by Use of Microcoil Equipment

Pawel Stanczak, Reto Horst, Pedro Serrano, Kurt Wüthrich
Journal of the American Chemical Society | 2009 | 10.1021/ja907842u

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Enhanced Covariance Spectroscopy from Minimal Datasets

Yanbin Chen, Fengli Zhang, Wolfgang Bermel, Rafael Brüschweiler
Journal of American Chemical Society | 2006 | 10.1021/ja065522e

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Monodisperse Protein-Based Glycopolymers via a Combined Biosynthetic and Chemical Approach

Ying Wang, Kristi L. Kiick
Journal of American Chemical Society | 2005 | 10.1021/ja055102+

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MaP Peptides:  Programming the Self-Assembly of Peptide-Based Mesoscopic Matrices

Maxim G. Ryadnov, Derek N. Woolfson
Journal of the American Chemical Society | 2005 | 10.1021/ja052972i

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Nanosecond Protein Dynamics:  First Detection of a Neutron Incoherent Spin−Echo Signal

Marie-Claire Bellissent-Funel, Roy Daniel, Dominique Durand, Michel Ferrand, John L. Finney, Stéphanie Pouget, Valérie Réat, Jeremy C. Smith
Journal of the American Chemical Society | 1998 | 10.1021/ja981329b

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Liposome Encapsulation of Conjugated Polyelectrolytes:  Toward a Liposome Beacon

An Thien Ngo, Pierre Karam, Elisa Fuller, Melanie Burger, Gonzalo Cosa
Journal of American Chemical Society | 2007 | 10.1021/ja076217b

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Multicomponent analysis with microbial sensors.

Volker Plegge, Christiane Zaborosch, M Slama, A C Konig, Klaus Riedel, Keith Baronian, Gotthard Kunze
2006

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Enzymatic Hydrogelation of Small Molecules

Zhimou Yang, Bing Xu, Gaolin Liang
Acc. Chem. Res. | 2008 | 10.1021/ar7001914

Enzymes, a class of highly efficient and specific catalysts in Nature, dictate a myriad of reactions that constitute various cascades in biological systems. Self-assembly, a process prevalent in Nature, also plays important roles in biology, from maintaining...

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Composite Layer-by-Layer (LBL) Assembly with Inorganic Nanoparticles and Nanowires

Nicholas A. Kotov, Sudhanshu Srivastava
Acc. Chem. Res. | 2008 | 10.1021/ar8001377

New assembly techniques are required for creating advanced materials with enough structural flexibility to be tuned for specific applications, and to be practical, the techniques must be implemented at relatively low cost. Layer-by-layer (LBL) assembly is a...

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Rational Design of Surface/Interface Chemistry for Quantitative in Vivo Monitoring of Brain Chemistry

Lanqun Mao, Ping Yu, Meining Zhang
Acc. Chem. Res. | 2012 | 10.1021/ar200196h

To understand the molecular basis of brain functions, researchers would like to be able to quantitatively monitor the levels of neurochemicals in the extracellular fluid in vivo. However, the chemical and physiological complexity of the central nervous system...

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A Mimicking Enzyme Analogue for Chemical Sensors

Annamalai Senthil Kumar, Jyh-Myng Zen
Acc. Chem. Res. | 2001 | 10.1021/ar000073n

An artificial enzyme analogue of Nafion/lead−ruthenium oxide pyrochlore (Py) chemically modified electrode (NPyCME) is synthesized by in situ precipitation through blocking of Nafion's hydrophilic zones. The catalytically active Py sites covered with a...

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Lariat Ether Receptor Systems Show Experimental Evidence for Alkali Metal Cation−π Interactions

Leonard J. Barbour, Jiaxin Hu, Riccardo Ferdani, George W. Gokel
Acc. Chem. Res. | 2002 | 10.1021/ar000093p

Cation−π interactions occur between cations and electron-rich species such as double bonds, triple bonds, and arenes. The π-electron system may be neutral or anionic, but the latter are less relevant to biology, at least so far as is currently known....

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FRET-Based Small-Molecule Fluorescent Probes: Rational Design and Bioimaging Applications

Weiying Lin, Kaibo Zheng, Lin Yuan, Sasa Zhu
Acc. Chem. Res. | 2013 | 10.1021/ar300273v

Fluorescence imaging has emerged as a powerful tool for monitoring biomolecules within the context of living systems with high spatial and temporal resolution. Researchers have constructed a large number of synthetic intensity-based fluorescent probes for...

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