With Tag: Biochemical Methods

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Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device

Klavs F. Jensen, Jungmin Lee, Woo Young Sim, Robert Langer, Andrea Adamo, Moungi G. Bawendi, Armon Sharei
Nano Lett. | 2012 | 10.1021/nl303421h

The ability to straightforwardly deliver engineered nanoparticles into the cell cytosol with high viability will vastly expand the range of biological applications. Nanoparticles could potentially be used as delivery vehicles or as fluorescent sensors to...


DNAzyme-Functionalized Au Nanoparticles for the Amplified Detection of DNA or Telomerase Activity

Itamar Willner, Tamara Niazov, Yi Xiao, Valeri Pavlov, Ron Gill
Nano Letters | 2004 | 10.1021/nl0491428

DNAzyme-functionalized Au−NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in the presence of hemin, H 2 O 2 , and luminol, the generation of...


Two-Photon 3D FIONA of Individual Quantum Dots in an Aqueous Environment

Fangfu Ye, Paul D. Simonson, Ruobing Zhang, Paul R. Selvin, Ido Golding, Ward Lopes, Tony Ng, Gilbert Fruhwirth, Eli Rothenberg
Nano Lett. | 2011 | 10.1021/nl201225r

We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36...


Imaginary Magnetic Tweezers for Massively Parallel Surface Adhesion Spectroscopy

Randall M. Erb, Benjamin B. Yellen, Stefan Zauscher, Ye Yang, Benjamin J. Wiley
Nano Lett. | 2011 | 10.1021/nl200189w

A massively parallel magnetic tweezer system has been constructed that utilizes the self-repulsion of colloidal beads from a planar interface via a magnetic dipole image force. Self-repulsion enables the application of a uniform magnetic force to thousands of...


Probing Single DNA Molecule Transport Using Fabricated Nanopores

Jiajun Gu, Peng Chen, Qiao Wang, Young-Rok Kim, Daniel Branton, Eric Brandin
Nano Letters | 2004 | 10.1021/nl048654j

Nanopores can serve as high throughput, single-molecule sensing devices that provide insight into the distribution of static and dynamic molecular activities, properties, or interactions. We have studied double stranded DNA electrophoretic transport dynamics...


A Single-Molecule Sensitive DNA Hairpin System Based on Intramolecular Electron Transfer

Hannes Barsch, Jens-Peter Knemeyer, Volker Buschmann, Thomas Heinlein, Oliver Piestert, Markus Sauer, Kenneth D. Weston
Nano Letters | 2003 | 10.1021/nl0341988

We describe a method for detection of sub-picomolar concentrations of DNA or RNA sequences using novel surface-immobilized DNA hairpins. Within the DNA hairpins a fluorophore is specifically quenched by guanosine residues in the complementary stem sequence...


Location-Specific Biological Functionalization on Nanotubes:  Attachment of Proteins at the Ends of Nanotubes Using Au Nanocrystal Masks

Lingtao Yu, Ipsita A. Banerjee, Hiroshi Matsui
Nano Letters | 2003 | 10.1021/nl034038w

We developed a novel method to immobilize proteins at a specific location on nanotubes. In this report, avidin was immobilized only at the ends of peptide nanotubes using Au nanocrystals as protective masks on the sidewalls of nanotubes. While the Au...


Preparation of Nucleic Acid Functionalized Carbon Nanotube Arrays

Jie Han, Jun Li, Lance Delzeit, M. Meyyappan, Alan M. Cassell, Cattien V. Nguyen
Nano Letters | 2002 | 10.1021/nl025689f

A vertically aligned carbon nanotube (CNT) array is fabricated as a nanoelectrode platform for biosensor development. Prior to chemical functionalization, metal catalyst particles at the ends of CNT are removed and the closed ends are opened. We find that the...


Frequency-Selective Rotation of Two-Particle Nanoactuators for Rapid and Sensitive Detection of Biomolecules

Andrea Ranzoni, Menno W. J. Prins, Jean J. H. B. Schleipen, Leo J. Van IJzendoorn
Nano Lett. | 2011 | 10.1021/nl200384p

We describe an optomagnetic bionanotechnologyfor rapidand sensitive solution-based affinity assays. Nanoactuators made from bioactive magnetic nanoparticles undergo rotational motion in the volume of a fluid under frequency-controlled magnetic actuation. The...


Identifying the Mechanism of Biosensing with Carbon Nanotube Transistors

Ethan D. Minot, Jaan Männik, Serge G. Lemay, Iddo Heller, Cees Dekker, Anne M. Janssens
Nano Lett. | 2007 | 10.1021/nl072996i

Carbon nanotube transistors have outstanding potential for electronic detection of biomolecules in solution. The physical mechanism underlying sensing however remains controversial, which hampers full exploitation of these promising nanosensors. Previously...


Translocation of Single-Wall Carbon Nanotubes Through Solid-State Nanopores

Adam R. Hall, Johannes M. Keegstra, Mark C. Hersam, Cees Dekker, Matthew C. Duch
Nano Lett. | 2011 | 10.1021/nl200873w

We report the translocation of individual single-wall carbon nanotubes (SWNTs) through solid-state nanopores. Single-strand DNA oligomers are used to both disperse the SWNTs in aqueous solution and to provide them with a net charge, allowing them to be driven...


Synthesis, Characterization, and Utility of Thermoresponsive Natural/Unnatural Product Macroligands for Affinity Chromatography

Ananthapadmanab Sivaramakrishnan, Min Zhou, Woon-Kai Low, Daniel Romo, David E. Bergbreiter, Chunmei Li, Jun O. Liu, Krishan Ponnamperuma
Org. Lett. | 2006 | 10.1021/ol062045w

The synthesis and characterization of thermoresponsive, water-soluble poly-N-isopropyl acrylamide (PNIPAM) derived macroligands displaying cyclosporin A (CsA) and dexamethasone (Dex) for use as novel affinity resins are described. Characterization of these...


Colorimetric Discrimination between Important Alkaloid Nuclei Mediated by a Bis-Salphen Chromophore

Arjan W. Kleij, Sander J. Wezenberg, Jordi Benet-Buchholz, Eduardo C. Escudero-Adán
Org. Lett. | 2008 | 10.1021/ol801167r

A new colorimetric method is presented that allows for simple and effective discrimination between (iso)quinoline nuclei pertinent to many alkaloid species. The method is based on the reversible incorporation of Zn 2+ ions into a bis-salphen chromophore...


Multi-Emissive Difluoroboron Dibenzoylmethane Polylactide Exhibiting Intense Fluorescence and Oxygen-Sensitive Room-Temperature Phosphorescence

Steven E. Kooi, Sarah J. Payne, Cassandra L. Fraser, Guoqing Zhang, Jianbin Chen, J. N. Demas
J. Am. Chem. Soc. | 2007 | 10.1021/ja0720255

Boron difluoride compounds are light emitting materials with impressive optical properties. Though their strong one- and two-photon absorption and intense fluorescence are well-known and exploited in molecular probes, lasers, and photosensitizers,...


Microarrays of Synthetic Heparin Oligosaccharides

Peter H. Seeberger, Jose L. De Paz, Christian Noti
J. Am. Chem. Soc. | 2006 | 10.1021/ja057584v

We present the first preparation of microarrays containing synthetic heparin oligosaccharides in order to elucidate the heparin−protein interactions involved in a variety of biological processes. For this purpose, we have developed a novel linker strategy...


Electrochemical “Switching” of Si(100) Modular Assemblies

Guillaume Le Saux, Michael James, J. Justin Gooding, Simone Ciampi, Katharina Gaus
J. Am. Chem. Soc. | 2011 | 10.1021/ja210048x

We report on a modular approach for producing well-defined and electrochemically switchable surfaces on Si(100). The switching of these surfaces is shown to change a Si(100) surface from resistant to cell adsorption to promoting cell adhesion. The...


A Simple Method to Distinguish Intermonomer Nuclear Overhauser Effects in Homodimeric Proteins with C2 Symmetry

Hiroshi Matsuo, Kylie J. Walters, Gerhard Wagner
J. Am. Chem. Soc. | 1997 | 10.1021/ja963309k



Sensitivity and Specificity of Metal Surface-Immobilized “Molecular Beacon” Biosensors

Todd D. Krauss, Benjamin L. Miller, Christopher M. Strohsahl, Hui Du, James Camera
J. Am. Chem. Soc. | 2005 | 10.1021/ja042482a

The separate developments of microarray patterning of DNA oligonucleotides, and of DNA hairpins as sensitive probes for oligonucleotide identification in solution, have had a tremendous impact on basic biological research and clinical applications. We have...


DNA-Based Magnetic Nanoparticle Assembly Acts as a Magnetic Relaxation Nanoswitch Allowing Screening of DNA-Cleaving Agents

Ralph Weissleder, Lee Josephson, Terence O'Loughin, J. Manuel Perez, F. Joseph Simeone
J. Am. Chem. Soc. | 2002 | 10.1021/ja017773n

Monodisperse magnetic nanoparticles conjugated with complementary oligonucleotide sequences self-assemble into stable magnetic nanoassemblies resulting in a decrease of the spin−spin relaxation times (T2) of neighboring water protons. When these...


Magneto-Switchable Bioelectrocatalysis

Itamar Willner, Ronit Hirsch, Eugenii Katz
J. Am. Chem. Soc. | 2000 | 10.1021/ja005523l


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