Site-directed ligand discovery

D. A. Erlanson , A. C. Braisted , D. R. Raphael , M. Randal , R. M. Stroud , E. M. Gordon , J. A. Wells

Add Review (press R) Confirm Authorship

Paper Rating

Paper Details

DOI 10.1073/pnas.97.17.9367

Journal Proceedings of the National Academy of Sciences
Location p 9367-9372, Volume 97, Issue 17, 2000
Publication Type journal-article

Tags escherichia coli, protein engineering, ligands, thymidylate synthase, enzyme, binding sites, drug design, molecular weight, infectious disease, thermodynamics, protein conformation, multidisciplinary
Publisher Link Click Here

Review

31 Jan 2021

Ken Hallenbeck

(1)

Important contribution to fragment hit-finding technologies

Logic / Design

Reproducibility

Impact

Transparency

Clarity

Versatility

Innovation

This paper by Erlanson and colleagues describes the disulfide tethering technique, which has proven to be a reproducible and versatile method for finding fragment hits. The main advance is the covalent targeting of the fragment library to a specific site on a protein surface....

View Detail / Comment

/paper/doi/10.1073/pnas.97.17.9367	0.000000	0.000000
INIT	0.014689	0.014689
_initView	0.022300	0.036989
_initViewBasic	0.005123	0.042112
_initSiteModules	0.001772	0.043884
_initRouters	0.008598	0.052482
pre dispatch _	0.034194	0.086676
LC::doiAction START	0.000205	0.086881
LC::doiAction END to showListing	0.287617	0.374498
LC::showListing START	0.000007	0.374505
LC::showListing get reviews START	0.011131	0.385636
ES Review Search START	0.024854	0.410490
ES Review Search END	0.005582	0.416072
LC::showListing get reviews END	0.005998	0.422070
Done	0.231964	0.654034

Loading...

Site-directed ligand discovery

D. A. Erlanson , A. C. Braisted , D. R. Raphael , M. Randal , R. M. Stroud , E. M. Gordon , J. A. Wells

Overall Rating

Review

Important contribution to fragment hit-finding technologies

Recently Reviewed

Has Cited

Cited by

Sign In With